基因總覽
SULT2A1（sulfotransferase family 2A member 1）是蛋白質編碼基因，產物屬於 sulfotransferase 1 家族。其蛋白質為硫酸基轉移酶，主要角色是將硫酸基轉移至多種小分子底物；已知底物包括類固醇、固醇、膽酸，以及帶有醇基或酚基的外源化合物。這類修飾可改變底物性質，與體內類固醇、脂質及膽酸的代謝調節相關。
基因異名與舊稱
異名取自 HGNC，可能包含歷史名稱；蛋白質名稱以 UniProt 註解為準。
染色體位置與基因結構
查看實際轉錄本與 exon 註解
| Transcript | Genomic coordinates | Exons |
|---|---|---|
| ENST00000222002 ★ | 47,870,467–47,886,315 | 6 |
| ENST00000903235 | 47,870,467–47,886,479 | 4 |
| ENST00000903236 | 47,870,467–47,886,479 | 5 |
| ENST00000903237 | 47,870,467–47,886,479 | 4 |
| ENST00000903239 | 47,870,467–47,886,479 | 2 |
| ENST00000986148 | 47,870,467–47,886,479 | 5 |
| ENST00001138582 | 47,870,467–47,886,479 | 6 |
| ENST00000986146 | 47,870,581–47,886,479 | 5 |
| ENST00000986154 | 47,870,686–47,886,479 | 4 |
| ENST00000903238 | 47,871,172–47,886,479 | 6 |
顯示 10/10 個蛋白質編碼轉錄本；canonical 優先。
帶型與著絲點依 Ensembl 座標繪製；核染色體長度以 GRCh38 第 1 號染色體為共同尺度。黃色標記表示本基因位置，細小區間加粗以利辨識；實際基因長度與 exon 見下方資料。
分子功能與生物學過程
SULT2A1 使用 3'-phospho-5'-adenylyl sulfate（PAPS）作為硫酸基供體，催化類固醇與膽酸的硫酸化；來源指出此作用發生於肝臟與腎上腺。其底物範圍涵蓋 pregnenolone、androsterone、DHEA、膽酸及 cholesterol，也包括具有醇基或酚基的多種外源化合物。反應將硫酸基接至底物，並產生 adenosine 3',5'-bisphosphate 與 H(+)。
此酵素可將 DHEA 轉為 DHEA 3-sulfate、pregnenolone 轉為 pregnenolone sulfate，也能硫酸化 androsterone。對膽酸類底物，反應產物包括 lithocholate 3-sulfate，以及多種膽酸及其 glyco、tauro 共軛形式的 3-sulfate。SULT2A1 也可催化 (24S)-hydroxycholesterol 的硫酸化，形成 24-sulfate 或 3-sulfate，並能進一步形成 3,24-disulfate。
硫酸化通常會提高化合物的水溶性，因而有助於其經腎臟排出；但來源也指出，這項修飾有時會使化合物生物活化，形成具活性的代謝物。因此，硫酸化的結果取決於底物，不能一概視為單純失活或排除。SULT2A1 參與類固醇與脂質恆定，也在膽酸代謝中促成 3-硫酸化膽酸的形成。
此外，依序列相似性推定，SULT2A1 可催化具強致癌性的多環芳香族芳基甲醇代謝活化；這項敘述屬於相似性推論。
Promoter 與基因調控
>SULT2A1 ENST00000222002 GRCh38 chr19:47886215-47887315 strand=-1 5′→3′ 相對 TSS：-1000 到 +100 bp；1101 bp 依轉錄本 TSS 擷取的基因組序列；TSS = 0，兩端包含，未驗證為 promoter CACTACACTCCAGCCTGGACGACAGAGTGAGACTCCATCTCAAAAAAAAAAAAAAAACAAACAAACACAT AATTAACACTTAAGGTTGGGTGCTGCCAATTCTTTGTGAAAATCCAAATATTATTAAGGAAACAGGGAGA TGCCACTACCTCTTGATTTTCCATCTAAAAACATACATGTTTATGCAAACAAATCTTTCCATATTCATAG TGACTTTTCAAGTATTTGAGTCTAAAGATTTTCATCTCACATTTTTATACCGTTTAAATTGTTCACAATT ATTACATACACGTCAGCCATCAACTAAAGTTGTACTTTAAAAATTTACTACAATATATACACTGCTAATA TTTGTACTTACTCTTTTTTTCTCTAGAGTGGAGGTATAATTGTGTGATACTTCAGAAATACAGATAAATG ATTCAAAAAGTCTCAGGGAGAATAATGTTTCTTTGATCGTGAATAACTGATTAGTAATTCTTGCCTATAT TTTCCTGAGAGCATATGACAAATGTTTCTAAGGTAACAAGATGAGAACAGATAAAGACTGTGTGGTCTTT TGGATTTGGAGAGACATATTTTAATTTTTAAATGCAGTTACAAATTATAATGTATTCATATTTGTACTTT CTGTTAAAATGCACGATTGCAGGATTATTTAGATTTTGTGTTTATGCTTGATGAAAAGCTTTGTTCTTGT TTTTAAGTTTGCACTCAAACCTTAAGAAATAAATTCACCCATATTATCAAAAAAAATATTTGTCCTCGTG TTTGTTATTCGATCTTGCAGTTCACTCTCAGGAACGCAAGCTCAGATGACCCCTAAAATGGTCTCTAGAT AAGTTCATGATTGCTCAACATCTTCAATCTTTTGAGTATGGGTCACATTATACCTCTCTTTATCAGCAAG TAAACTTTACAACAAACATGTGACATGCTGGGACAAGGTTAAAGATCGTTTTATCCTTGCTGTAAAAGCT GATCTGCCTGTAGCTGCCACAGCCTCCAGCGGTGGCTACAGTTGAAACCCTCACACCACGCAGGAAGAGG TCATCATCATGTCGGACGATTTCTTATGGTTTGAAGGCATAGCTTTCCCTA
EPDnew 實驗支持 promoter／TSS · 1 筆
| EPDnew ID | TSS（1-based） | 股向 | 來源顯示區間 |
|---|---|---|---|
| SULT2A1_1 ↗ | 19:47,886,315 | 負股 | 47,886,305–47,886,364 |
EPDnew human v006 · UCSC hg38，GRCh38/hg38，透過 UCSC 取得。TSS 具有 CAGE／RAMPAGE 等實驗資料支持；任意擷取的上下游區間仍需另行判讀調控功能，不能視為整段皆經功能驗證。
EPD 封存狀態 ↗ · UCSC 資料說明 ↗ · EPD team, SIB Swiss Institute of Bioinformatics; UCSC Genome Browser · CC BY 4.0
查看已取得的調控／motif 註解
· motif · 47886358–47886371 · motif
· motif · 47886457–47886466 · motif
· motif · 47889599–47889610 · motif
蛋白質結構域與序列
蛋白質三維結構
細胞內定位
SULT2A1 的定位資料指出其位於細胞質。
深綠色圓點：來源記載的蛋白質定位；數量不表示含量。
動物細胞結構示意；器官大小與數量不按比例。點選細胞構造可查看定位註解。
交互作用與訊號網絡
拖曳節點後放開可帶動位置變化；點選夥伴查看證據。位置與連線長度不代表結合強度，圖中未推定夥伴彼此之間的關係。
更多交互作用夥伴 · 已取得 3 個夥伴
- NIF3L1Q9GZT8 · UniProt INTERACTION · 3 experiments
- STAT3P40763 · UniProt INTERACTION · 2 experiments
- SULT1B1O43704 · UniProt INTERACTION · 6 experiments
SULT2A1 可形成同源二聚體。
疾病與遺傳關聯
Contextual Functional Evidence Matrix
矩陣以「功能主張」為列，以細胞情境、干預條件、量測結果與證據來源為欄，方便比較同一功能在不同條件下的支持情形。只有含情境與實驗資訊的紀錄會列入矩陣；一般功能註解另列於下方。
功能註解總覽 · 22 筆
| 功能面向 | 功能敘述 | 證據類型 | 可查核來源 |
|---|---|---|---|
| 功能 | SULT2A1 是硫酸基轉移酶，利用 3'-phospho-5'-adenylyl sulfate（PAPS）提供硫酸基，在肝臟與腎上腺催化類固醇及膽酸硫酸化。其底物包括 pregnenolone、androsterone、DHEA、膽酸、cholesterol，以及帶有醇基或酚基的多種外源化合物。硫酸化通常增加水溶性並促進腎臟排出，但也可能使化合物生物活化。該酵素參與類固醇、脂質恆定及膽酸 3-硫酸化；另依序列相似性推定，可代謝活化具致癌性的多環芳香族芳基甲醇。 | 含實驗來源 | 證據與來源Sulfotransferase that utilizes 3'-phospho-5'-adenylyl sulfate (PAPS) as sulfonate donor to catalyze the sulfonation of steroids and bile acids in the liver and adrenal glands. Mediates the sulfation of a wide range of steroids and sterols, including pregnenolone, androsterone, DHEA, bile acids, cholesterol and as well many xenobiotics that contain alcohol and phenol functional groups. Sulfonation increases the water solubility of most compounds, and therefore their renal excretion, but it can also result in bioactivation to form active metabolites. Plays an important role in maintening steroid and lipid homeostasis. Plays a key role in bile acid metabolism, mediating formation of 3-sulfated bile acids. In addition, catalyzes the metabolic activation of potent carcinogenic polycyclic arylmethanols (By similarity) PMID 14573603 ↗ · PMID 18042734 ↗ · PMID 19589875 ↗ · PMID 20102295 ↗ · PMID 21187059 ↗ · PMID 2268288 ↗ · PMID 29671343 ↗ · PMID 7678732 ↗ · PMID 7854148 ↗ |
| 分子活性 | an alcohol + 3'-phosphoadenylyl sulfate = an alkyl sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源an alcohol + 3'-phosphoadenylyl sulfate = an alkyl sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 18042734 ↗ · PMID 19589875 ↗ · PMID 20102295 ↗ · PMID 21187059 ↗ · PMID 2268288 ↗ · PMID 7678732 ↗ · PMID 7854148 ↗ |
| 分子活性 | (24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 24-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源(24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 24-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 19589875 ↗ |
| 分子活性 | (24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源(24S)-hydroxycholesterol + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 19589875 ↗ |
| 分子活性 | (24S)-hydroxycholesterol 24-sulfate + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3,24-disulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源(24S)-hydroxycholesterol 24-sulfate + 3'-phosphoadenylyl sulfate = (24S)-hydroxycholesterol 3,24-disulfate + adenosine 3',5'-bisphosphate + H(+) PMID 19589875 ↗ |
| 分子活性 | 3beta-hydroxyandrost-5-en-17-one + 3'-phosphoadenylyl sulfate = dehydroepiandrosterone 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源3beta-hydroxyandrost-5-en-17-one + 3'-phosphoadenylyl sulfate = dehydroepiandrosterone 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 14573603 ↗ · PMID 18042734 ↗ · PMID 19589875 ↗ · PMID 20102295 ↗ · PMID 21187059 ↗ · PMID 2268288 ↗ · PMID 7678732 ↗ |
| 分子活性 | pregnenolone + 3'-phosphoadenylyl sulfate = pregnenolone sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源pregnenolone + 3'-phosphoadenylyl sulfate = pregnenolone sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 29671343 ↗ |
| 分子活性 | androsterone + 3'-phosphoadenylyl sulfate = androsterone 3alpha-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源androsterone + 3'-phosphoadenylyl sulfate = androsterone 3alpha-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 14573603 ↗ · PMID 18042734 ↗ |
| 分子活性 | lithocholate + 3'-phosphoadenylyl sulfate = lithocholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源lithocholate + 3'-phosphoadenylyl sulfate = lithocholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ · PMID 2268288 ↗ |
| 分子活性 | glycolithocholate + 3'-phosphoadenylyl sulfate = glycolithocholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源glycolithocholate + 3'-phosphoadenylyl sulfate = glycolithocholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | taurolithocholate + 3'-phosphoadenylyl sulfate = taurolithocholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源taurolithocholate + 3'-phosphoadenylyl sulfate = taurolithocholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ · PMID 2268288 ↗ |
| 分子活性 | deoxycholate + 3'-phosphoadenylyl sulfate = deoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源deoxycholate + 3'-phosphoadenylyl sulfate = deoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | glycodeoxycholate + 3'-phosphoadenylyl sulfate = glycodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源glycodeoxycholate + 3'-phosphoadenylyl sulfate = glycodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | taurodeoxycholate + 3'-phosphoadenylyl sulfate = taurodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源taurodeoxycholate + 3'-phosphoadenylyl sulfate = taurodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | ursodeoxycholate + 3'-phosphoadenylyl sulfate = ursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源ursodeoxycholate + 3'-phosphoadenylyl sulfate = ursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | glycoursodeoxycholate + 3'-phosphoadenylyl sulfate = glycoursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源glycoursodeoxycholate + 3'-phosphoadenylyl sulfate = glycoursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | tauroursodeoxycholate + 3'-phosphoadenylyl sulfate = tauroursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源tauroursodeoxycholate + 3'-phosphoadenylyl sulfate = tauroursodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | chenodeoxycholate + 3'-phosphoadenylyl sulfate = chenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源chenodeoxycholate + 3'-phosphoadenylyl sulfate = chenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | glycochenodeoxycholate + 3'-phosphoadenylyl sulfate = glycochenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源glycochenodeoxycholate + 3'-phosphoadenylyl sulfate = glycochenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 分子活性 | taurochenodeoxycholate + 3'-phosphoadenylyl sulfate = taurochenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) | 含實驗來源 | 證據與來源taurochenodeoxycholate + 3'-phosphoadenylyl sulfate = taurochenodeoxycholate 3-sulfate + adenosine 3',5'-bisphosphate + H(+) PMID 20102295 ↗ |
| 交互作用 | Homodimer | 含實驗來源 | |
| 細胞定位 | SULT2A1 位於細胞質。 | 含實驗來源 |
核酸與蛋白質序列
AGCCTCCAGCGGTGGCTACAGTTGAAACCCTCACACCACG40CAGGAAGAGGTCATCATCATGTCGGACGATTTCTTATGGT80TTGAAGGCATAGCTTTCCCTACTATGGGTTTCAGATCCGA120AACCTTAAGAAAAGTACGTGATGAGTTCGTGATAAGGGAT160GAAGATGTAATAATATTGACTTACCCCAAATCAGGAACAA200ACTGGTTGGCTGAGATTCTCTGCCTGATGCACTCCAAGGG240GGATGCCAAGTGGATCCAATCTGTGCCCATCTGGGAGCGA280TCACCCTGGGTAGAGAGTGAGATTGGGTATACAGCACTCA320GTGAAACGGAGAGTCCACGTTTATTCTCCTCCCACCTCCC360CATCCAGTTATTCCCCAAGTCTTTCTTCAGTTCCAAGGCC400AAGGTGATTTATCTCATGAGAAATCCCAGAGATGTTTTGG440TGTCTGGTTATTTTTTCTGGAAAAACATGAAGTTTATTAA480GAAACCAAAGTCATGGGAAGAATATTTTGAATGGTTTTGT520CAAGGAACTGTGCTATATGGGTCATGGTTTGACCACATTC560ATGGCTGGATGCCCATGAGAGAGGAGAAAAACTTCCTGTT600ACTGAGTTATGAGGAGCTGAAACAGGACACAGGAAGAACC640ATAGAGAAGATCTGTCAATTCCTGGGAAAGACGTTAGAAC680CCGAAGAACTGAACTTAATTCTCAAGAACAGCTCCTTTCA720GAGCATGAAAGAAAACAAGATGTCCAATTATTCCCTCCTG760AGTGTTGATTATGTAGTGGACAAAGCACAACTTCTGAGAA800AAGGTGTATCTGGGGACTGGAAAAATCACTTCACAGTGGC840CCAAGCTGAAGACTTTGATAAATTGTTCCAAGAGAAGATG880GCAGATCTTCCTCGAGAGCTGTTCCCATGGGAATAACGTC920CAAAACACTCTGGATCTTATATGGAGAATGACATTGATTC960cDNA 使用 DNA 字母 T；mRNA 模式改以 U 顯示。轉錄本保留已註解的非編碼區，未另外加入 poly(A) 尾；非編碼 RNA 的參考轉錄本也可能仍需後續加工。數字為所選序列的 1-based 位置。長序列以黃色捲軸瀏覽，可複製或下載完整轉錄本與蛋白質；基因組 DNA 隨捲動取得所需範圍。