基因總覽
SHH 是蛋白質編碼基因，產物 sonic hedgehog signaling molecule 屬於 hedgehog family。前驅蛋白長 462 個胺基酸，帶有 N 端訊號胜肽；其結構包含可自我切割的前驅區域，並可形成 Sonic hedgehog protein N-product（ShhN）與 C-product（ShhC）。ShhN 經脂質修飾後成為具有訊號功能的形態素，ShhC 則在內質網中降解。此蛋白的主要生物學角色與發育期間的組織圖樣建立相關。
基因異名與舊稱
異名取自 HGNC，可能包含歷史名稱；蛋白質名稱以 UniProt 註解為準。
染色體位置與基因結構
查看實際轉錄本與 exon 註解
| Transcript | Genomic coordinates | Exons |
|---|---|---|
| ENST00000297261 ★ | 155,799,980–155,812,463 | 3 |
| ENST00000430104 | 155,800,040–155,809,072 | 4 |
顯示 2/2 個蛋白質編碼轉錄本；canonical 優先。
帶型與著絲點依 Ensembl 座標繪製；核染色體長度以 GRCh38 第 1 號染色體為共同尺度。黃色標記表示本基因位置，細小區間加粗以利辨識；實際基因長度與 exon 見下方資料。
分子功能與生物學過程
SHH 前驅蛋白在內質網中進行自我蛋白水解，切分為 ShhN 與 ShhC；同時，前驅蛋白 C 端具有膽固醇轉移酶活性，可將膽固醇共價接到新生成的 ShhN C 端。這些步驟的證據標示為相似性推論。ShhN 還會接受額外脂質修飾，形成雙脂質化產物；ShhC 則在內質網中降解。資料描述的反應涉及蛋白質、膽固醇與氫離子，生成帶有 C 端甘胺酸膽固醇酯的蛋白質及 N 端半胱胺醯蛋白質。
雙脂質化的 ShhN 是形態素，能結合 patched 受體 PTCH1 或 PTCH2，解除 patched 對 smoothened（SMO）的抑制，進而啟動 smoothened signaling pathway，並促進標的基因轉錄。沒有 SHH 時，patched 會抑制 SMO 的構成性訊號活性。這種受體調控使 SHH 訊號得以參與發育中的組織圖樣形成。
ShhN 在中樞神經系統與肢體發育的圖樣建立中具有重要角色；依相似性推論，它可誘導神經管與體節的腹側細胞命運，參與發育中肢芽前後軸圖樣建立，並對軸突導引不可或缺。這些作用將 SHH 訊號與神經及肢體發育過程中的細胞命運和空間組織連結起來；相關生物學作用的部分描述屬於相似性推論。
Promoter 與基因調控
>SHH ENST00000297261 GRCh38 chr7:155812363-155813463 strand=-1 5′→3′ 相對 TSS：-1000 到 +100 bp；1101 bp 依轉錄本 TSS 擷取的基因組序列；TSS = 0，兩端包含，未驗證為 promoter GTTCCCCCAGGGCCCCTGACCCCTTGGGCTGCAGCCCCTAGGACTGGTGGCTCCTGTGCCTGAAGACTGC TGGCCTGCTGAGCCCCCACACAAGGCCAGCTTTGCAAGGAGCCTGAATTCCACATTCAGCTCACCCCTGG CTCTGCACTTTGCTCTGATCGATCGTGGGGGTTGTGTGAGCACAGCTTCGCCCACTCGCCTGCCCCCCCC CCAACACCCTGCAATGGCCTCCACACACCCAGCGCACACACTGGCCCCCACTCTCTCCCCTGTGCTGGGG TGCAGCCCCCAGACCTGCTTTGCCCATCCACCCTGACGCCCGCAGTTCACTCTGTAACTGCCCTTGAGCA CGCCTCCATCCCATTTGCAGAGCTCTACAAAAAAAAAAATGTATTAATTGAAGCACAATAAAAGAGCAGT ATTTTTACATTTTTCTAAGGCACTTAAAGCATTTCAAATACCAGTGCTTTAAGTTATGGTCACTCATAAT TATCCTCGGTCCACAGAGGCTGAGTCTTCAGGAGGGGCTCAGTCCTCTTTCTTCAAAATGCAGGGAGAGC CAGCCTGCACTATAAAGTCCCTGGGGAAGAAAAATTAAATAACAGCTGGGCAGCTGTGCCCCTCAAATGT GTGAGCGACTCTGTGCTTGATGACTGAAGCTTGCGGGGGTTTAAACTGTCCTTTAAAAACTTAAGTTGGT TTTAACTGAAGCACCTACCATTCCAGGGAAAGTCAGGGGGAAACGCAAGGATGTACACAAAGCCGGGAGT AACTGCTGTTACCACAAGACATGCGCTCCTCCCTCCCGCCCACCTTTATCTTAGGAAAGATTTTTTTTTT TTTAACTCGAATCCTTCAGGTGAAAATAAAGCAACAGCAGCAACAGAAAAAAAAAACGTAGTCTTCTTCA GGGTTAACATCAGAAGACAAGCTTGTGCGGCTTTGGCCAATCAGATGCGCCCCTGGCAGCTATAATATAG TGCTAAAGGCAGCCTCTCTCACAAGCTCTCCAGGCTTGCTACCATTTAAAATCAGACTCTTTTTGTCTTT TGATTGCTGTCTCGCGACCCAACTCCGATGTGTTCCGTTATCAGCGGCCGG
EPDnew 實驗支持 promoter／TSS · 3 筆
| EPDnew ID | TSS（1-based） | 股向 | 來源顯示區間 |
|---|---|---|---|
| SHH_3 ↗ | 7:155,809,163 | 負股 | 155,809,153–155,809,212 |
| SHH_2 ↗ | 7:155,812,277 | 負股 | 155,812,267–155,812,326 |
| SHH_1 ↗ | 7:155,812,463 | 負股 | 155,812,453–155,812,512 |
EPDnew human v006 · UCSC hg38，GRCh38/hg38，透過 UCSC 取得。TSS 具有 CAGE／RAMPAGE 等實驗資料支持；任意擷取的上下游區間仍需另行判讀調控功能，不能視為整段皆經功能驗證。
EPD 封存狀態 ↗ · UCSC 資料說明 ↗ · EPD team, SIB Swiss Institute of Bioinformatics; UCSC Genome Browser · CC BY 4.0
查看已取得的調控／motif 註解
ENSR7_53CMH63 · CTCF_binding_site · 155808579–155808600 · regulatory
ENSR7_CKGG2 · enhancer · 155810055–155811360 · regulatory
ENSR7_86KNKQ · promoter · 155811537–155811738 · regulatory
ENSR7_949SRN · promoter · 155812453–155812784 · regulatory
ENSR7_86KNMK · enhancer · 155814598–155814847 · regulatory
ENSR7_949SSG · promoter · 155815123–155815590 · regulatory
ENSR7_86KNN4 · enhancer · 155815591–155815739 · regulatory
ENSR7_949SSM · enhancer · 155815857–155816141 · regulatory
· motif · 155807836–155807849 · motif
· motif · 155808011–155808020 · motif
· motif · 155808011–155808020 · motif
· motif · 155808065–155808080 · motif
· motif · 155808092–155808102 · motif
· motif · 155808180–155808193 · motif
· motif · 155808581–155808597 · motif
· motif · 155809046–155809059 · motif
· motif · 155809318–155809331 · motif
· motif · 155809424–155809434 · motif
· motif · 155809620–155809630 · motif
· motif · 155809785–155809795 · motif
· motif · 155813307–155813322 · motif
· motif · 155815407–155815416 · motif
· motif · 155815501–155815510 · motif
蛋白質結構域與序列
參考蛋白質長度：462 aa
sonic hedgehog signaling molecule 屬於 hedgehog family，全長 462 個胺基酸，N 端訊號胜肽位於第 1–23 位。第 32–38 位含 Cardin-Weintraub motif。第 279–302 位與第 395–414 位均註記為無序區域。前驅蛋白另具有自我蛋白水解及膽固醇轉移酶活性。
蛋白質三維結構
細胞內定位
前驅 Sonic hedgehog protein 位於內質網膜與 Golgi apparatus 膜，並與 HHAT 在這兩處共定位。 成熟的雙脂質化 ShhN 則錨定於細胞膜並可形成多聚體；SCUBE2 可促進其由細胞表面釋出，使其參與遠距訊號傳遞。
深綠色圓點：來源記載的蛋白質定位；數量不表示含量。
細胞外定位：圓點散佈於細胞周圍，與細胞膜保持距離。
動物細胞結構示意；器官大小與數量不按比例。點選細胞構造可查看定位註解。
交互作用與訊號網絡
拖曳節點後放開可帶動位置變化；點選夥伴查看證據。位置與連線長度不代表結合強度，圖中未推定夥伴彼此之間的關係。
更多交互作用夥伴 · 已取得 2 個夥伴
- HHIPQ96QV1 · UniProt INTERACTION · 10 experiments
- PTCH1Q13635 · UniProt INTERACTION · 2 experiments · 2 筆來源紀錄
SHH 可形成多聚體，並與 HHIP、GPC3 及 HHATL/GUP1 互作；HHATL/GUP1 會負向調節 HHAT 對 SHH N 端的棕櫚醯化。SCUBE2 作為載體促進 ShhN 離開細胞表面，DISP1 則透過膽固醇錨定與 SHH 互作。BOC 與 CDON 形成異源二聚體，並與 ShhN 作為共同受體；GAS1 也具有 ShhN 共同受體角色。
疾病與遺傳關聯
影響 SHH 的變異與 Microphthalmia/Coloboma 5、Holoprosencephaly 3 及 Solitary median maxillary central incisor 有關，分別涉及眼部形成、前腦分隔異常與單一上顎正中門齒先天缺失。另有多種肢體異常與 SHH 調控改變相關：LMBR1 intron 5 的變異可破壞 SHH 長距離順式調控元件，造成 SHH 表現模式改變或異位表現，涉及 Triphalangeal thumb with polysyndactyly、Preaxial polydactyly 2 及 Hypoplasia or aplasia of tibia with polydactyly。Laurin-Sandrow syndrome 則與包含 LMBR1 intron 5 中 ZRS 調控序列的 16–75 kb 重複，以及異常肢體 SHH 表現有關。
Contextual Functional Evidence Matrix
矩陣以「功能主張」為列，以細胞情境、干預條件、量測結果與證據來源為欄，方便比較同一功能在不同條件下的支持情形。只有含情境與實驗資訊的紀錄會列入矩陣；一般功能註解另列於下方。
功能註解總覽 · 14 筆
| 功能面向 | 功能敘述 | 證據類型 | 可查核來源 |
|---|---|---|---|
| 功能 | SHH 前驅蛋白是形態素的前體，可啟動 smoothened signaling pathway，並參與發育期間多種組織圖樣形成。其 C 端在內質網中具有自我蛋白水解及膽固醇轉移酶活性，產生 ShhN 和 ShhC，並將膽固醇共價接至 ShhN 的 C 端；這些加工機制標示為相似性推論。ShhN 經額外脂質修飾後發揮形態素作用，ShhC 則在內質網降解。 | 含實驗來源 | 證據與來源Precursor of sonic hedgehog, a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. The C-terminal part of the precursor displays an autoproteolysis and a cholesterol transferase activity, resulting (1) in the cleavage of the full-length protein into two parts, Sonic hedgehog protein N-product and C-product (ShhN and ShhC, respectively) and (2) covalent attachment of a cholesterol moiety to the C-terminus of the newly generated ShhN (By similarity). Both autoproteolysis and a cholesterol transferase activities occur in the endoplasmic reticulum (By similarity). Following additional lipidation, ShhN acts as a morphogen, while ShhC is degraded in the endoplasmic reticulum (By similarity) PMID 29954986 ↗ · PMID 29995851 ↗ · PMID 30139912 ↗ · PMID 31127104 ↗ · PMID 31548691 ↗ |
| 功能 | 雙脂質化的 ShhN 是形態素，會結合 PTCH1 或 PTCH2，解除 patched 對 SMO 的抑制，啟動 smoothened signaling pathway 並促進標的基因轉錄；缺少 SHH 時，patched 會抑制 SMO 的構成性活性。依相似性推論，ShhN 參與中樞神經系統與肢體圖樣建立、神經管和體節腹側細胞命運、肢芽前後軸圖樣，以及軸突導引。 | 含實驗來源 | 證據與來源The dually lipidated sonic hedgehog protein N-product (ShhN) is a morphogen that activates the smoothened signaling pathway, and which is essential for a variety of patterning events during development. Acts by binding to the patched receptor (PTCH1 or PTCH2), relieving smoothened (SMO) inhibition by patched, activating the smoothened signaling pathway and transcription of target genes. In the absence of SHH, patched represses the constitutive signaling activity of SMO. ShhN is widely expressed and plays a key role in the patterning of the central nervous system and limb development (By similarity). Induces ventral cell fate in the neural tube and somites (By similarity). Involved in the patterning of the anterior-posterior axis of the developing limb bud (By similarity). Essential for axon guidance (By similarity) PMID 10753901 ↗ · PMID 29995851 ↗ · PMID 30139912 ↗ · PMID 31127104 ↗ · PMID 31548691 ↗ · PMID 31555730 ↗ · PMID 24522195 ↗ · PMID 24863049 ↗ |
| 分子活性 | glycyl-L-cysteinyl-[protein] + cholesterol + H(+) = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein] | 推論支持 | 證據與來源glycyl-L-cysteinyl-[protein] + cholesterol + H(+) = [protein]-C-terminal glycyl cholesterol ester + N-terminal L-cysteinyl-[protein] |
| 交互作用 | SHH 與 HHATL/GUP1、HHIP 和 GPC3 互作；其中 HHATL/GUP1 會負向調節 HHAT 媒介的 SHH N 端棕櫚醯化。HHIP 與 SHH 的互作有文獻支持，HHATL/GUP1 和 GPC3 的互作則標示為相似性推論。 | 含實驗來源 | 證據與來源Interacts with HHATL/GUP1 which negatively regulates HHAT-mediated palmitoylation of the SHH N-terminus (By similarity). Interacts with HHIP. Interacts with glypican GPC3 (By similarity) PMID 19561609 ↗ |
| 交互作用 | SHH 可形成多聚體，並與 SCUBE2、DISP1、BOC、CDON 及 GAS1 互作。SCUBE2 是載體，可促進雙脂質化 ShhN 從細胞表面釋出；DISP1 透過膽固醇錨定與 SHH 互作。BOC 和 CDON 形成異源二聚體，作為 ShhN 共同受體的描述屬相似性推論；GAS1 亦作為 ShhN 共同受體。 | 含實驗來源 | 證據與來源Multimer. Interacts with SCUBE2; SCUBE2 acts as a carrier to promote release of the dual-lipidated sonic hedgehog protein N-product (ShhN) from the cell surface. Interacts with DISP1 via its cholesterol anchor. Interacts with BOC and CDON; BOC and CDON form a heterodimer that acts as a coreceptor for ShhN (By similarity). Interacts with GAS1; GAS1 acts as a coreceptor for ShhN PMID 19561609 ↗ · PMID 22677548 ↗ · PMID 22902404 ↗ · PMID 24522195 ↗ · PMID 35231446 ↗ |
| 細胞定位 | Sonic hedgehog protein 位於內質網膜與 Golgi apparatus 膜，並與 HHAT 在內質網及 Golgi apparatus 膜共定位。 | 推論支持 | 證據與來源Sonic hedgehog protein; Endoplasmic reticulum membrane; Golgi apparatus membrane; Co-localizes with HHAT in the ER and Golgi membrane PMID 18534984 ↗ |
| 細胞定位 | ShhN 是分泌型產物，透過脂質錨定固定於細胞膜並形成多聚體。SCUBE2 可使其由產生細胞表面釋出，支持遠距形態素訊號傳遞；之後 ShhN 轉交給 CDON-BOC 異源二聚體及 GAS1 共同受體，再到達 PTCH1 或 PTCH2。 | 含實驗來源 | 證據與來源Sonic hedgehog protein N-product; Secreted; Cell membrane / Lipid-anchor; The dual-lipidated sonic hedgehog protein N-product (ShhN) is firmly tethered to the cell membrane where it forms multimers. Further solubilization from the cell surface is achieved by SCUBE2 carrier, which promotes its release from producing cells, allowing it to act as a morphogen for distant signaling. ShhN is then transferred to the CDON-BOC heterodimer and GAS1 coreceptors to reach the patched receptor (PTCH1 or PTCH2) PMID 24342078 ↗ · PMID 24522195 ↗ · PMID 35231446 ↗ |
| 疾病關聯 | Microphthalmia/Coloboma 5 是眼部形成異常，嚴重程度可從單眼偏小到雙側完全缺少眼組織，也可能伴隨角膜或水晶體混濁、視網膜和脈絡膜疤痕等異常。眼部缺損可源於視杯、視柄形態發育異常及胎兒裂隙融合異常；此疾病由影響 SHH 的基因變異造成。 | 含實驗來源 | 證據與來源Microphthalmia/Coloboma 5 MCOPCB5 A disorder of eye formation, ranging from small size of a single eye to complete bilateral absence of ocular tissues. Ocular abnormalities like opacities of the cornea and lens, scaring of the retina and choroid, and other abnormalities may also be present. Ocular colobomas are a set of malformations resulting from abnormal morphogenesis of the optic cup and stalk, and the fusion of the fetal fissure (optic fissure). The disease is caused by variants affecting the gene represented in this entry MIM: 611638 PMID 12503095 ↗ |
| 疾病關聯 | Holoprosencephaly 3 是前腦發育時未能正確分隔為左右半球的結構異常，疾病在遺傳與臨床表現上具有異質性。嚴重程度從伴隨嚴重臉部異常的無葉型，到沒有腦部畸形、僅有輕微顱顏缺陷的葉型或微型表現皆有；多數病例看似偶發，但也有明確的體染色體顯性遺傳案例。此病由影響 SHH 的基因變異造成。 | 含實驗來源 | 證據與來源Holoprosencephaly 3 HPE3 A form of holoprosencephaly, a structural anomaly of the brain in which the developing forebrain fails to correctly separate into right and left hemispheres. It is a genetically and clinically heterogeneous disorder with a wide spectrum of severity, ranging from alobar holoprosencephaly with severe facial abnormalities, such as cyclopia and proboscis, to mild forms that include lobar or microform holoprosencephaly, without cerebral malformations and with mild craniofacial defects. The majority of HPE3 cases are apparently sporadic, although clear examples of autosomal dominant inheritance have been described. The disease is caused by variants affecting the gene represented in this entry MIM: 142945 PMID 10441331 ↗ · PMID 10556296 ↗ · PMID 11479728 ↗ · PMID 15107988 ↗ · PMID 15221788 ↗ · PMID 15942952 ↗ · PMID 15942953 ↗ · PMID 16282375 ↗ · PMID 17001669 ↗ · PMID 19603532 ↗ · PMID 8896572 ↗ · PMID 9302262 ↗ |
| 疾病關聯 | Solitary median maxillary central incisor 是罕見牙齒異常，特徵為先天缺少一顆上顎中門齒；此疾病與影響 SHH 的基因變異有關。 | 含實驗來源 | 證據與來源Solitary median maxillary central incisor SMMCI Rare dental anomaly characterized by the congenital absence of one maxillary central incisor. The disease is caused by variants affecting the gene represented in this entry MIM: 147250 PMID 11471164 ↗ · PMID 15103725 ↗ |
| 疾病關聯 | Triphalangeal thumb with polysyndactyly 為體染色體顯性症候群，肢體發育期間 SHH 表現模式改變與其前軸及後軸異常相關。致病變異位於 LMBR1 的 intron 5，會破壞 SHH 的長距離順式調控元件；疾病關聯描述為 SHH 基因參與致病過程。 | 含實驗來源 | 證據與來源Triphalangeal thumb with polysyndactyly TPTPS Autosomal dominant syndrome. It is characterized by a wide spectrum of pre- and post-axial abnormalities due to altered SHH expression pattern during limb development. The gene represented in this entry is involved in disease pathogenesis. SHH expression is altered due to disease-causing variants located in intron 5 of LMBR1 disrupt a long-range, cis-regulatory element of SHH MIM: 190605 PMID 12837695 ↗ · PMID 18417549 ↗ |
| 疾病關聯 | Preaxial polydactyly 2 的多指表現涉及遠端指骨重複，拇指通常可對掌且掌骨正常。LMBR1 intron 5 的變異會破壞 SHH 長距離順式調控元件，造成異常、異位的 SHH 表現並帶來病理後果；此基因被描述為參與疾病致病過程。 | 含實驗來源 | 證據與來源Preaxial polydactyly 2 PPD2 Polydactyly consists of duplication of the distal phalanx. The thumb in PPD2 is usually opposable and possesses a normal metacarpal. The gene represented in this entry is involved in disease pathogenesis. Mutations located in intron 5 of LMBR1 disrupt a long-range, cis-regulatory element of SHH and result in abnormal, ectopic SHH expression with pathological consequences MIM: 174500 PMID 12837695 ↗ |
| 疾病關聯 | Hypoplasia or aplasia of tibia with polydactyly 為體染色體顯性疾病，特徵是脛骨發育不全或缺如並伴隨多指。LMBR1 intron 5 的變異會破壞 SHH 長距離順式調控元件，造成異常、異位的 SHH 表現並導致病理後果；SHH 被描述為參與疾病致病過程。 | 含實驗來源 | 證據與來源Hypoplasia or aplasia of tibia with polydactyly THYP An autosomal dominant disease characterized by hypoplastic or absent tibia, and polydactyly. The gene represented in this entry is involved in disease pathogenesis. Mutations located in intron 5 of LMBR1 disrupt a long-range, cis-regulatory element of SHH and result in abnormal, ectopic SHH expression with pathological consequences MIM: 188740 PMID 19847792 ↗ · PMID 24965254 ↗ |
| 疾病關聯 | Laurin-Sandrow syndrome 是罕見體染色體顯性疾病，可有手或足多指併指、足部鏡像重複、鼻部缺陷，以及腓骨與脛骨身分辨別異常；部分患者沒有鼻部異常。此病與涉及 LMBR1 intron 5 中 ZPA 調控序列（ZRS）的 16–75 kb 重複有關，並造成肢體 SHH 表現異常及病理後果。 | 含實驗來源 | 證據與來源Laurin-Sandrow syndrome LSS A rare autosomal dominant disorder characterized by polysyndactyly of hands and/or feet, mirror image duplication of the feet, nasal defects, and loss of identity between fibula and tibia. Some patients do not have nasal abnormalities (segmental Laurin-Sandrow syndrome). The gene represented in this entry is involved in disease pathogenesis. Abnormal SHH limb expression with pathological consequences is caused by duplications (16-75 kb) involving the ZPA regulatory sequence (ZRS), a SHH long-range cis-regulatory element, located in LMBR1 intron 5 MIM: 135750 PMID 24456159 ↗ |
核酸與蛋白質序列
ACAAGCTCTCCAGGCTTGCTACCATTTAAAATCAGACTCT40TTTTGTCTTTTGATTGCTGTCTCGCGACCCAACTCCGATG80TGTTCCGTTATCAGCGGCCGGCAGCCTGCCATTCCAGCCC120CTGTCTGGGTGGGGAGCGGTGGAGAGTCCCCCGCAGCCGC160GGCGGGCAAGGTTATATAGGAAGAGAAAGAGCGAGGCAGC200CAGCGAGGGAGAGAGCGAGCGGGCGAGCCGGAGCGAGGAA240GGGAAAGCGCAAGAGAGAGCGCACACGCACACACCCGCCG280CGCGCACTCGCGCACGGACCCGCACGGGGACAGCTCGGAA320GTCATCAGTTCCATGGGCGAGATGCTGCTGCTGGCGAGAT360GTCTGCTGCTAGTCCTCGTCTCCTCGCTGCTGGTATGCTC400GGGACTGGCGTGCGGACCGGGCAGGGGGTTCGGGAAGAGG440AGGCACCCCAAAAAGCTGACCCCTTTAGCCTACAAGCAGT480TTATCCCCAATGTGGCCGAGAAGACCCTAGGCGCCAGCGG520AAGGTATGAAGGGAAGATCTCCAGAAACTCCGAGCGATTT560AAGGAACTCACCCCCAATTACAACCCCGACATCATATTTA600AGGATGAAGAAAACACCGGAGCGGACAGGCTGATGACTCA640GAGGTGTAAGGACAAGTTGAACGCTTTGGCCATCTCGGTG680ATGAACCAGTGGCCAGGAGTGAAACTGCGGGTGACCGAGG720GCTGGGACGAAGATGGCCACCACTCAGAGGAGTCTCTGCA760CTACGAGGGCCGCGCAGTGGACATCACCACGTCTGACCGC800GACCGCAGCAAGTACGGCATGCTGGCCCGCCTGGCGGTGG840AGGCCGGCTTCGACTGGGTGTACTACGAGTCCAAGGCACA880TATCCACTGCTCGGTGAAAGCAGAGAACTCGGTGGCGGCC920AAATCGGGAGGCTGCTTCCCGGGCTCGGCCACGGTGCACC960cDNA 使用 DNA 字母 T；mRNA 模式改以 U 顯示。轉錄本保留已註解的非編碼區，未另外加入 poly(A) 尾；非編碼 RNA 的參考轉錄本也可能仍需後續加工。數字為所選序列的 1-based 位置。長序列以黃色捲軸瀏覽，可複製或下載完整轉錄本與蛋白質；基因組 DNA 隨捲動取得所需範圍。